Microscopes
A note from Anna:
"The number of users for the microscopes in lab has increased quite a bit. To ensure efficiency, I have created two booking calendars: for the katyperryscope and the nissanmicrascope. If you think of better puns we can rename them.
You will get access to these calendars once you’ve been ‘trained’ on the relevant microscope.
See image above. The one one in SEB606 (left) is katyperryscope, the other one (right) is nissanmicrascope. The nissanmicrascope has the micromanipulators on it and is currently downstairs"
Rules:
Be considerate
Be organised
Be courteous
Be reasonable
If you need it for longer than 10 mins, please BOOK TIME
If you need it for longer than 1 hour, please BOOK TIME at least 1 day in advance
If you need it for less than 10 mins, check the calendar before bringing your sample into lab
Protocol:
BOOK the microscope as per rules above
Dispose of chemistry gloves in the bin underneath nissanmicrascope.
Wash your hands before using the microscopes with ungloved hands OR use clean gloves.
Do not use immersion oil unless you are using the 100x objectives. Use sparingly and please clean with specialised lens wipes before you leave. NEVER use kimwipes never ever.
Turn everything off once you’re finished. Checklist:
White LED
Fluorescence LED
XY/joystick stage controller
Camera
Heating stage (if using)
When imaging:
Be gentle! Do not mash the objectives into glass, the objectives are expensive and can break.
Do not move the position of the condenser knob unless you realign it (and if you don't know what these words mean - don't move it).
Leave the area cleaner than when you arrived.
Throw your samples out
Close pipette tip boxes
You may need to get pipettors from next door and/or tips from the far end of 605 (our lab benches are the ones closest to Hilmer). If so please return the pipettors themselves back to 605. The tips can stay in 606.
For data acquisition, saving temporarily onto a local drive is recommended for faster read/write speeds. For longer term storage, please store your data on the Z drive. This is backed up regularly and can be accessed off-campus. For instructions on the group share, please see the New Researcher Checklist.
For cleaning the objective (or any optics):
Refer to this guide: https://www.youtube.com/watch?v=-RetyUAWGc0
Waste disposal:
Dispose of glass slides in the sharps bin
Dispose of pipette tips etc in the solid waste bin
Dispose of other hazardous waste somewhere you know how to deal with them (maybe that’s your own lab, or in 605)
If you encounter any problems or issues contact Anna. If you have any suggestions please also contact Anna.