Microscopes

A note from Anna:


"The number of users for the microscopes in lab has increased quite a bit. To ensure efficiency, I have created two booking calendars: for the katyperryscope and the nissanmicrascope. If you think of better puns we can rename them.

You will get access to these calendars once you’ve been ‘trained’ on the relevant microscope.

See image above. The one one in SEB606 (left) is katyperryscope, the one in SEB629 (right) is nissanmicrascope. The nissanmicrascope has the micromanipulators on it"


Rules:

  • Be considerate

  • Be organised

  • Be courteous

  • Be reasonable

  • If you need it for longer than 10 mins, please BOOK TIME

  • If you need it for longer than 1 hour, please BOOK TIME at least 1 day in advance

  • If you need it for less than 10 mins, check the calendar before bringing your sample into lab

Protocol:

  • BOOK the microscope as per rules above

  • Dispose of chemistry gloves in the bin underneath nissanmicrascope.

  • Wash your hands before using the microscopes with ungloved hands OR use clean gloves.

  • Do not use immersion oil unless you are using the 100x objectives. Use sparingly and please clean with specialised lens wipes before you leave. NEVER use kimwipes never ever.

  • Turn everything off once you’re finished. Checklist:

    • White LED

    • Fluorescence LED

    • XY/joystick stage controller

    • Camera

    • Heating stage (if using)

  • When imaging:

    • Be gentle! Do not mash the objectives into glass, the objectives are expensive and can break.

    • Do not move the position of the condenser knob unless you realign it (and if you don't know what these words mean - don't move it).

  • Leave the area cleaner than when you arrived.

    • Throw your samples out

    • Close pipette tip boxes

  • You may need to get pipettors from next door and/or tips from the far end of 605 (our lab benches are the ones closest to Hilmer). If so please return the pipettors themselves back to 605. The tips can stay in 606.


Waste disposal:

  • Dispose of glass slides in the sharps bin

  • Dispose of pipette tips etc in the solid waste bin

  • Dispose of other hazardous waste somewhere you know how to deal with them (maybe that’s your own lab, or in 605)

If you encounter any problems or issues contact Anna. If you have any suggestions please also contact Anna.